P65

Mycobacterium bovis (M. bovis) is the etiological agent of bovine tuberculosis (bTB). The infectious disease represents an economic problem and a threat to animals' public health due to the zoonosis of the disease. Vaccination of cattle with the BCG vaccine is offering promising results. But still available diagnostic methods in developed countries with low budgets are limited. Although genotyping, for example, Spoligotyping, is helpful to determine genetic structure diversity among the strains, it can not provide information about the evolutionary origin and circulation, vital for effective control, movement, and surveillance of cattle worldwide.  Herein, the objective of the work is to analyze the evolutionary origin and circulation of M. bovis strains of Mexico in assembled genomes (n= 429). Phylogenomics and Comparative genomics analysis show that M. bovis strains from these regions cluster in the same clade and that genomes (n = 169) of M. bovis from Mexico clustered closely with Canada, England, and Korean M. bovis strains. From the data, we can conclude that Mexican M. bovis isolates have been circulated and spread in the country and from other countries to Mexico.