P78

Macrophages play a very important role in mycobacterial infections, but also in the processes of tumor progression. The so-called tumor-associated macrophages (M2) are characterized by activating an anti-inflammatory and oncogenic response. Reversion of M2 macrophages to M1 macrophages, with inflammatory and antitumor properties, is possible due to the plasticity of macrophages. Mycobacterium bovis BCG (BCG) is currently used as a vaccine for tuberculosis and as an antitumor agent in patients with non-invasive non-muscular bladder cancer. Its role in the tumor microenvironment is not entirely defined. Our study aimed to evaluate the immunomodulatory potential of BCG and Mycolicibacterium brumae, a non-pathogenic mycobacterium with antitumor capacities, in the phenotypic processes of polarization and reversion of M1 and M2 macrophages and their effect in the cell migration and invasion of bladder cancer cells. Our results indicate that both BCG and M. brumae are capable of polarizing and reversing macrophages towards an antitumor phenotype contributing to the diminishing of the migratory and invasiveness capacities of T24 and J82 bladder cancer cells.