P098

1:Facultad de Química. Universidad Juárez del Edo de Durango; 2:Instituto Multidisciplinario de Ciencias AVICENA. Torreon, Coahuila. Mexico; 3:Unidad Academica de Ciencias Biológicas. Universidad Autónoma de Zacatecas

Bovine Tuberculosis (bTB) is a zoonotic disease caused by Mycobacterium bovis that affects cattle and wild animal species, thus contributing to its dissemination and transmission. It is a threat to ecology, agriculture, and human health. The problem aggravates because diagnostic tools have been based on classic methods, the tuberculin test (PPD) and histopathology, due to the high costs of the molecular test implemented in some reference laboratories. The composition of the cell wall is among the factors that play a role in antimicrobial resistance. The waxy cell wall of pathogenic mycobacteria of Mtb is structured of three structural types of MAs: 1) the alpha-, 2) the methoxy, and c) the keto mycolic acids (alpha-, M-, and K-MAs). The peculiarity of these types of MAs is that both the keto and methoxy derivatives not only enhance the pathogenicity of Mtb but also allow it to modulate the host immune response. In previous work, we isolated Mycobacterium bovis from the nostrils of cattle in the region of Zacatecas, Mexico. From the strains of M. bovis analyzed, mostly they have type I mycolates, polar and apolar lipids. Therefore, we sought to dissect in detail the types of MAs and lipids of the cell wall of M. bovis isolates from nearby regions, using, in addition to thin layer chromatography (TLC), spectroscopic techniques (eg, RMN, mass spectrometry).