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Whole Genome Sequence of Mycolicibacterium parafortuitum Panama NTM 1 isolated from a cattle farm in Panama

F Guizado(1) E Hernández(1) A Ramírez(2) E del Pilar Santamaría(1) H Moris(1) J Chen(1) P Mislov(1) V Batista(3) A Prescilla(2) J Ku(3) F Acosta(3) A Llanes(3) A Goodridge(3) N Ortiz de Moreno(2)

1:School of Medicine, Universidad de Panamá, Ciudad de Panamá, Panamá; 2:Department of Microbiology of the School of Medicine, Universidad de Panamá, Panamá.; 3:Centro de Biología Molecular y Celular de Enfermedades, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología (INDICASAT-AIP); Ciudad del Saber, Panamá.

Non-tuberculosis mycobacteria (NTM) are environmental organisms that can infect humans and animals. They can be found in soil, dust, and water. Mycolicibacterium parafortuitum is a rapidly growing NTM. We aim to characterize and annotate a de novo genome assembly of Mycolicibacterium parafortuitum isolated from a cattle farm in Panama.

The feces, perianal zone, and milk samples were decontaminated with (N-acetylcysteine + NaOH 2%) and cultivated on Lowenstein-Jensen medium. The isolate was analyzed using biochemical and microbiological methods.  Next-generation sequencing was performed using Illumina´s platform. Phylogenetic analysis was conducted using 16S rRNA sequence. Our results confirmed the isolate's identity as Mycolicibacterium parafortuitum Panama NTM 1 strain. Phylogenetic analysis demonstrated a close relationship with Mycolicibacterium parafortuitum strain JCM 6367. We observed a high conservation in gene content, including synteny between their genomes.

These results allowed us to confirm the identity of the Mycolicibacterium parafortuitum NTM 1 strain at the genomic level. The genomic characteristic provides novel information on the isolate of Mycolicibacterium parafortuitum NTM 1 in Panama. In addition, it provides the basis for future research on pathogenicity, evolution, and molecular biomarker studies of veterinary and public health importance.

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